This Handbook is the first of its kind addressing gender issues in health in five countries of the South Asian Region, namely: Bangladesh, India, Pakistan and Sri Lanka. Adopting a social determinant of health perspective and an intersectionality and diversity lens, the Handbook illustrates the multi-layered complexities of gender, health, and well-being from the diverse perspectives and lived experiences in different South Asian countries. It includes studies on under-researched and often invisible marginalized populations, such as LGBTQI populations, urban poor, persons living with disability, migrant and conflict-affected populations. It represents the voices of the elderly, adolescents, and young people. It goes beyond analyzing the problem of gender inequities in health, and present examples of gender-transformative policies, programmes and social movement action. It is an essential resource for researchers, policy-makers, students in public health and community-based organizations involvedin research, policies, or programs related to sex work, public health, social justice and gender-based violence.