This book constitutes the thoroughly refereed proceedings of the 16th International Conference on Transport Systems Telematics, TST 2016, held in Katowice-Ustrón, Poland, in March 2016. The 37 full and 5 short papers presented in this volume were carefully reviewed and selected from 110 submissions. They present and organize the knowledge from within the field of intelligent transportation systems, the specific solutions applied in it and their influence on improving efficiency of transport systems.