This handbook presents recent advances and offers a comprehensive reference resource covering the developments in and applications of macrocyclic supramolecular assembly, with a focus on their construction, structural characters and biological functions. The main topics addressed include: 

1. Construction and structure of macrocyclic supramolecular assembly – key building blocks, construction methods, structural motifs, and stimuli responsive control
2. Approach and technology – controllable synthesis, molecular recognition, spectral and thermodynamic study, supramolecular assembly at interfaces, orthogonal self-assembly, the supramolecular organic framwork (SOF), molecular induced aggregation, supramolecule assisted 3D printing, theoretical calculation and molecular simulation 
3. Biological applications – chemical and biological sensing, theranostic tools, molecule/ion channels, drug/gene delivery, supramolecule assisted biomolecule production, supramolecule assisted transmembrane transport, supramolecule assisted immunity regulation, supramolecule-based medicinal drug, etc.