This book constitutes the thoroughly refereed post-conference proceedings of the 16th International Workshop on Coalgebraic Methods in Computer Science, CMCS 2022, colocated with ETAPS 2022, held in Munich, Germany, in April 2022.

The 9 revised full papers were carefully reviewed and selected from 12 submissions. The papers cover a wide range of topics in the theory, logics, and applications of coalgebras.