These proceedings are based on the international conference Approximation Theory XVI held on May 19–22, 2019 in Nashville, Tennessee. The conference was the sixteenth in a series of meetings in Approximation Theory held at various locations in the United States.  Over 130 mathematicians from 20 countries attended.  The book contains two longer survey papers on nonstationary subdivision and Prony’s method, along with 11 research papers on a variety of topics in approximation theory, including Balian-Low theorems, butterfly spline interpolation, cubature rules, Hankel and Toeplitz matrices, phase retrieval, positive definite kernels, quasi-interpolation operators, stochastic collocation, the gradient conjecture, time-variant systems, and trivariate finite elements. The book should be of interest to mathematicians, engineers, and computer scientists working in approximation theory, computer-aided geometric design, numerical analysis, and related approximation areas.