Fungi are an essential, fascinating and biotechnologically useful group of organisms with an incredible biotechnological potential for industrial exploitation. As a result of this steadily increased interest in fungi, the methods needed for their isolation, identification, their genetic manipulation, and monitoring of gene expression and protein production, have become refined and complemented. All techniques available in the area of fungal biology viz. protocols for sampling protozoan fungal analogues to entomopathogenic fungi; from fungi growing on and in animals and plants to mushrooms and truffles; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; applications in health and agricultural industries and bioinformatics tools in fungi will be covered and complied in this book. This book will serve as a first source for traditional methods of mycology as well as modern molecular techniques. This book shouldbe on the bookshelf of every serious practitioner of mycology in academia, industry, and government, and in clinical laboratories. It will serve as a rich resource on methods for seasoned professionals as well as for students at all levels. This book presents an inventory of techniques and methods that are currently in use for studying fungi by mycologists.