This book aims to promote the interaction among the scientific community to discuss applications of CS/AI with an interdisciplinary character, exploring the interactions between sub-areas of CS/AI, Bioinformatics, Chemoinformatics and Systems Biology. The success of Bioinformatics in recent years has been prompted by research in Molecular Biology and Molecular Medicine in several initiatives. This year’s technical program presents both high quality and diversity, with contributions in well-established and evolving areas of research. The PACBB’24 technical program has selected 22 full papers in the main track and, as in past editions, it will be special issues in ranked journals. This symposium is organized by the BISITE Research Group of the University of Salamanca (Spain). The author would like to thank all the contributing authors, the members of the Program Committee, National Associations (AEPIA, APPIA), and the sponsors (AIR Institute).