The Present book is the second version revised from CELL ENGINEERING (Second Edition), and was selected as a national teaching material for Chinese higher education in the 11the Five-Year Plan. This book comprises three parts: fundamentals of cell engineering, plant cell engineering, and animal cell engineering. Every effort has been made to comprehensively, systematically and concisely present the principles as well as techniques in relation to cell engineering. It is not only suitable as a text book for undergraduates majoring in bioengineering, biotechnology, medicine and pharmacy, agriculture etc., but also suitable as a reference book for cell engineering researchers.