This fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding of the potential of stem cells and the regulatory mechanisms controlling their fate, particularly through the parallel advances in nanotechnologies and their applications in biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.