This volume provides a
collection of protocols and approaches for the creation of novel ligand binding
proteins, compiled and described by many of today's leaders in the field of
protein engineering. Chapters focus on modeling protein ligand binding
sites, accurate modeling of protein-ligand conformational sampling, scoring of
individual docked solutions, structure-based design program such as ROSETTA,
protein engineering, and additional methodological approaches. Examples of applications
include the design of metal-binding proteins and light-induced ligand binding
proteins, the creation of binding proteins that also display catalytic
activity, and the binding of larger peptide, protein, DNA and RNA
ligands. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding
known pitfalls.