This new edition of Natural Products Isolation has been not only significantly updated to reflect recent advances in the field, but has also been expanded with three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. The book includes powerful cutting-edge techniques and simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Molecular Biology(t) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.