This book covers the characterization of nano-biomaterials in tissue repair and regeneration which are materials designed to interact with biological systems to restore and regenerate damaged tissues. These materials serve as scaffolds that mimic the natural extracellular matrix (ECM), providing structural support and biochemical cues necessary for cellular activities. The physical and chemical properties of biomaterials are tailored to specific applications, such as bone regeneration, cartilage repair, wound healing, and organ transplantation. Biomaterials influence cell behavior and tissue formation by controlling factors like mechanical strength, porosity, and surface topography. This book provides an overview of the topic from the biomaterials’ characterization point of view and is suitable for students, academics, and clinical practitioners who are interested in the advancements of regenerative medicine.