Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique, including isolation techniques for DNA and RNA, gene cloning, PCR, DNA sequencing, ELISA and cell culture. These protocols all includes a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.
- Provides step-by-step instructions to guide researchers in carrying out a range of molecular biology techniques
- Includes details of instrumentation, reagents and buffers necessary for each experiment
- Features diagrams to help illustrate the procedures and techniques covered