This book's pragmatic approach helps researchers in drug discovery to develop biologically relevant assays. It informs the development of unique biomarkers, new protein drugs, and the development and validation of immunoassays. The coverage focuses on practical aspects of bioanalysis using various platforms including capillary electrophoresis, mass spectrometry, as well as different ligand binding assay formats. By integrating the techniques of bioanalysis, exploration of the results, and validation of the methods, it provides a benchmark educational tool for professionals.