This third edition of A Practical Guide to Molecular Cloning provides complete updates to all of the protocols, and incorporates much new material to cover a broader range of biomolecular techniques. It covers the key information required for students to perform cloning of any DNA fragment and eventually study its expression products. It also serves as an excellent comprehensive guide for any researcher using molecular techniques. Protocols in this established manual have been used successfully to characterize cellular localization, nature, and function of gene products in both prokaryotes and eukaryotes. This book is the most accessible laboratory manual for those who want to understand why techniques work and how best to apply them.
* Presents reliable, accurate, and clear stepwise protocols * Includes all new coverage of genomics, proteomics (identification of targets and characterization of functional domains), PCR applications such as mutagenesis, and microarray technology and applications * Discusses the biochemistry underlying commonly seen kits, in order that the reader will be able to use the kits to solve non-classical problems * Includes new chapters on basic methods in microbiology, basic methods in cellular biology, methods for studying protein-protein interactions, SAGE, phage display, and methods for modifying the host genome * Includes numerous tables and an appendix of companies and useful websites