Experimental Process Biotechnology is one of the basic requirements for Biochemical Engineering, Bioprocess Engineering and Biotechnology. Theoretical basis of this course encampusing analytical bio-chemistry, microbiology, cell biology,biochemical and chemical engineering sciences, general and applied molecular biology, plant and animal cell culture engineering etc. is now well founded. This laboratory manual containing protocols is prepared on the basis of my personal learning exposures at Jadavpur University, Kolkata, experiences and supervision of laboratory work in microbial and enzyme engineering and technology conducted at Biochemical Engineering Research Centre, Deptt of Biochemical Engineering & Biotechnology, IIT Delhi, Fermentation technology laboratory, Osaka University, Japan and other places.