In this fully updated and revised 2nd edition of "Gene Therapy Protocols", leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, "Gene Therapy Protocols, 2nd Edition", offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application.