This lab manual emphasizes the basic principles of diagnostic microbiology for students preparing to enter the allied health field. Students are led through a series of exercises that allow them to learn basic microbiology techniques and practice safety in the laboratory and hospital environment. It is primarily oriented toward meeting the interests and needs of those who will be directly involved in patient care and wish to learn how microbiological principles should be applied in the practice of their professions.

The authors articulate the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. Exercises illustrate the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilization. The role of the health professional is highlighted in regard to the appropriate collection of clinical specimens and application of aseptic and disinfectant techniques as they relate to patient care.