This manual can be utilized by undergraduate and postgraduate students in various fields of biological sciences. It offers detailed, step-by-step guidance for conducting laboratory experiments. This manual will also be helpful for researchers and students planning experiments in a molecular biology laboratory.

The authors have extensive experience in molecular biology and are actively engaged in research and teaching in this area. At the end of each experiment, a set of questions has been included to help students better understand the topic. This manual also includes a set of objective questions related to molecular diagnostics that will be beneficial for both theoretical and competitive exams.