Drug-involved women are especially vulnerable to HIV infection through multiple routes of drug- and sex-related transmission from a primary male partner. With the support of the National Institutes of Health (NIH), National Institute on Drug Abuse (NIDA), researchers at the National Development and Research Institutes and Louisiana State University have developed an HIV counseling and testing intervention designed to address the unique needs of substance-using women in heterosexual relationships. This evaluation version of the manual describes the content and administration of the intervention in detail. The effectiveness of this program for reducing HIV risk is currently under clinical investigation. This manual is intended for researchers and service providers who wish to review and evaluate HIV prevention programs designed for women at high risk for HIV.