In
addition to updating important core techniques this third edition presents new
chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and
pericyte isolation techniques, spheroid and arterial ring based in vitro
assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols,
Third Edition also guides readers through new protocols on chorioallantoic
membrane models, corneal pocket assays to assess angio- and lymphangiogenesis,
models of muscle angiogenesis, and use of zebrafish embryos to study vascular
angiogenesis and senescence. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.



Authoritative
and practical, Angiogenesis Protocols, Third Edition provides not only a
practical handbook for key techniques, but also an informative and enjoyable
read for all those interested, no matter how directly, in angiogenesis.