This volume contains the proceedings of the International Workshop on Tropical and Idempotent Mathematics, held at the Independent University of Moscow, Russia, from August 26-31, 2012. The main purpose of the conference was to bring together and unite researchers and specialists in various areas of tropical and idempotent mathematics and applications. This volume contains articles on algebraic foundations of tropical mathematics as well as articles on applications of tropical mathematics in various fields as diverse as economics, electroenergetic networks, chemical reactions, representation theory, and foundations of classical thermodynamics. This volume is intended for graduate students and researchers interested in tropical and idempotent mathematics or in their applications in other areas of mathematics and in technical sciences.