This volume presents the proceedings of the Seventh International Symposium on Resonance Ionization Spectroscopy and its Applications, presenting an overview of current research in the field. The topics include ultrasensitive techniques, femtosecond RIS and its biological applications, RIS for molecular physics and surface analysis, new laser sources and applications, atomic RIS, molecular RIS, and RIS applied to nuclear and particle physics. It should be useful for researchers in spectroscopy and atomic, molecular, and optical physics.