This second edition expands on the first edition with new chapters describing methods for studying
cell movement, molecular components involved in chemotaxis, spatiotemporal
dynamics of signaling components, and quantitative modeling, as well as several
updated chapters from the first edition. Various methods to investigate
directional cell growth and movements are presented in Chapters 1-20. These
chapters contains experimental procedures to visualize and measure migration
behaviors of different kinds of organisms, including chemotropism in the
budding yeast; cell growth and migration of D. discoideum; border cell
migration in Drosophila; chemotaxis of mouse and human neutrophils; and
HIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,
Second Edition also contains microscopy procedures for studying breast cancer
cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes
with Chapters 21-27 describing methods that measure spatiotemporal dynamics of
signaling components involved in chemotaxis; introduce imaging techniques, such
as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of
experimentally generated chemoattractant gradients. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.

Cutting edge and thorough,
Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for
anyone who is interested in the diverse methodologies that are propelling
chemotaxis research forward.