Kinases belong to one of the most biologically
significant enzyme classes. The development of
analytical techniques for characterization of kinase
activity, in particular at a global scale, is a
central priority for proteomic and cell biology
researchers.
In order to facilitate global analysis of cellular
phosphorylation, a new paradigm of microarray
technology which focuses on analysis of total
cellular kinase activity, kinome, has emerged in the
past few years.
As the specificity of many kinases is dictated
primarily by recognition of residues immediately
surrounding the site of phosphorylation a logical
methodology is to employ peptides
representing these immediate sequences as
experimental substrates. Microarray chips carrying
hundreds of such substrate targets have been
developed for human kinome analysis, however,
lack of similar tools for species outside research
mainstream has limited kinome analysis in
these species. This work describes a methodology to
create species specific peptide arrays. A case study
is carried out to analyze Toll Like Receptor (TLR)
signaling pathways in bovine monocytes.