This volume contains the proceedings of the ICTS program Knot Theory and Its Applications (KTH-2013), held from December 10-20, 2013, at IISER Mohali, India. The meeting focused on the broad area of knot theory and its interaction with other disciplines of theoretical science. The program was divided into two parts. The first part was a week-long advanced school which consisted of minicourses. The second part was a discussion meeting that was meant to connect the school to the modern research areas. This volume consists of lecture notes on the topics of the advanced school, as well as surveys and research papers on current topics that connect the lecture notes with cutting-edge research in the broad area of knot theory.