When the Civil War broke out, women answered the call for help. They broke away from their traditional roles and served in many capacities, some of them even going so far as to disguise themselves as men and enlist in the army. Estimates of such women enlistees range from 400 to 700. About 60 women soldiers were known to have been killed or wounded.

More than sixty women who fought or who served the Union or Confederacy in other ways are featured. Among them are Sarah Thompson, the Union spy and nurse who brought down the famous raider John Hunt Morgan; Elizabeth Van Lew, the Union spy instrumental in the largest prison break of the war; Sarah Malinda Blalock, who fought for the Confederacy as a soldier and then for the Union as a guerrilla raider; Dr. Mary Walker, a doctor for the Union and the only woman to receive the Congressional Medal of Honor for Civil War service; and Jennie Hodgers, the longest serving woman soldier (and the only woman to receive a soldier's pension).