Arguably the first resource to tackle this subject, this book explores the newest tools for succeeding in synthetic biology projects. It demonstrates how to systematically construct models from scratch using synthetic protein, enzyme, or nucleic acid parts. The authors discuss approaches for part characterization, the integration of proteomic/transcriptomic data, modeling the chassis, and the debugging of designed circuits to fine tune their behavior. The book includes concrete examples, methods and available open-source resources for design, and supplementary material on the author’s web site and an accompanying CD.