Biting midges such as some Culicoides species can be a public health or veterinary problem, as vectors of disease or as the cause of severe discomfort, especially in coastal areas. This pictorial atlas presents 292 wing photographs of 145 described and 120 undescribed species of Culicoides biting midges from the Australasian biogeographic region. For 16 species, photographs of intraspecific pattern variants are included, some of them rare in collections. The atlas provides a species checklist for Australasian Culicoides and a guide to specific differences in wing patterns, venation and the distribution and form of macrotrichia. Morphometric data for described species are tabled to aid confirmation of the identity of slide-mounted specimens. The practical application of this Atlas is enhanced by the inclusion of photographs of the recognised undescribed species workers may encounter.