This book constitutes the refereed proceedings of the 9th International Conference on Machines, Computations, and Universality, MCU 2022, held in Debrecen, Hungary, in August/September 2022. The 10 revised full papers presented were carefully reviewed and selected from 18 submissions. MCU explores computation in the setting of various discrete models (Turing machines, register machines, cellular automata, tile assembly systems, rewriting systems, molecular computing models, neural models, concurrent systems, etc.) and analog and hybrid models (BSS machines, infinite time cellular automata, real machines, quantum computing).