Flow cytometry is one of the most powerful analytics and preparative techniques in cell biology. This book details six of the most useful topics in flow cytometry derived from six lectures from recognized experts in the field. Each lecture is accompanied by expert commentary, all of which is written in an easy to read style. The cutting-edge chapters cover a wide array of topics including designing a flow cytometry experiment, rare event analysis, functional assays, assays of cell proliferation, solid tissues, and imaging flow cytometry. Each topic is carefully discussed for its current relevance in the field, its importance in biomedical research, and the frequency of its misuse in the scientific literature.