Apiary inspectors and beekeepers must be able to recognize bee diseases and parasites and to differentiate the serious diseases from the less important ones. This handbook describes laboratory techniques used to diagnose diseases and other abnormalities of the honey bee and to identify parasites and pests. Emphasis is placed on the techniques used by the U.S. Department of Agriculture Bee Research Laboratory. Included are directions for submitting, through APHIS-PPQ or state regulators, samples of suspected Africanized honey bees for identification of subspecies. Also included are directions for sending diseased brood and adult honey bees for diagnosis of bee disease.