This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.