This book constitutes revised selected papers from the 13th International Conference on Data Integration in the Life Sciences, DILS 2018, held in Hannover, Germany, in November 2018.The 5 full, 8 short, 3 poster and 4 demo papers presented in this volume were carefully reviewed and selected from 22 submissions. The papers are organized in topical sections named: big biomedical data integration and management; data exploration in the life sciences; biomedical data analytics; and big biomedical applications.