The refereed proceedings of the 24th International Conference on Applications and Theory of Petri Nets, ICATPN 2003, held in Eindhoven, The Netherlands, in June 2003. The 25 revised full papers presented together with 6 invited contributions were carefully reviewed and selected from 77 submissions. All current issues on research and development in the area of Petri nets are addressed, in particular concurrent systems design and analysis, model checking, networking, business process modeling, formal methods in software engineering, agent systems, systems specification, systems validation, discrete event systems, protocols, and prototyping.