This book constitutes the proceedings of the 16th International Conference on Web Information Systems and Applications, WISA 2019, held in Qingdao, China, in September 2019.  The 39 revised full papers and 33 short papers presented were carefully reviewed and selected from 154 submissions. The papers are grouped in topical sections on machine learning and data mining, cloud computing and big data, information retrieval, natural language processing, data privacy and security, knowledge graphs and social networks, blockchain, query processing, and recommendations.