The authoritative guide on protein purification—now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:



High-resolution reversed-phase liquid chromatography


Electrophoresis in gels


Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients


Affinity ligands from chemical and biological combinatorial libraries


Membrane separations


Refolding of inclusion body proteins from E. coli


Purification of PEGylated proteins


High throughput screening techniques in protein purification


The history of protein chromatography