Retaining valuable information from the first edition, including fermentation kinetics and flux control analysis, this new edition addresses numerous newly important topics. New chapters explore the production of primary and secondary metabolites such as amino acids, nucleotides, and vitamins as well as antibiotics, immunosuppressants, and anti-tumor agents. The authors examine advances in enzyme and co-factor engineering, cell immobilization, and sensor technology. Packed with examples and industry case studies, the text is accessible to academic and industry researchers, as well as students in applied microbiology, and pharmaceutical biotechnology.