PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications. This book is intended for researchers at all levels in labs involved in molecular human genetics, mammalian genetics (mouse, rat, etc), molecular microbiologists, molecular parasitologists, biochemists who use molecular biology techniques, and some molecular diagnostic labs.