This volume is a collection of
research methods, techniques, and approaches to investigate the molecular
biology of West Nile Virus (WNV). Chapters in the book cover many facets of
WNV, such as propagation and titration of WNV in vero cells, examination of WNV
neuroinvasion and neuropathogenesis in the central nervous system of a murine
model, field surveillance methods for WNV, and in vitro and in vivo
blood-brain barrier models to study WNV pathogenesis. A brief introduction,
along with a discussion on WNV laboratory safety are also included. Written in
the highly successful Methods in Molecular Biology series format,
chapters includes introduction to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, West Nile Virus will interest virologists,
molecular biologists, microbiologists, and other scientists working with WNV.
This book is a comprehensive guide for those conducting research in the field
of WNV biology, including life cycles, pathogenesis, impact of infection, and
possible treatment/prevention development.